zd 7288  (Bio-Techne corporation)

Journal: bioRxiv

Article Title: Developmental mechanisms contributing to non-linear firing dynamics in spinal motoneurons of the postnatal mouse

doi: 10.64898/2026.03.12.711366

Figure Lengend Snippet: (A) Representative voltage clamp traces illustrating the H-current measured in response to a 10-mV hyperpolarizing voltage step applied from -60 mV before (black) and after application of the HCN channel blocker ZD7288 (10 µM) in 10 delayed firing motoneurons obtained from mice during the third postnatal week (P14-20). (B) ZD7288 eliminates H current measured at -70 mV. (C) Representative traces of the membrane potential and repetitive firing during a triangular depolarizing current ramp before (black) and after (red) application of ZD7288. ZD7288 increased recruitment-derecruitment hysteresis (D; Delta I) and produced a shift from Type 4 firing hysteresis in all cells to the emergence of a 5 th firing type characterized by self sustained firing (E). ZD7288 decreased the current at firing onset on the ascending limb of the ramp (F) decreased the current of firing offset on the descending limb of the ramp (G). Hyperpolarizing current was needed to terminate self-sustained firing elicited after triangular current ramps (H) or brief depolarizing current steps (I) in the presence of ZD7288. J) Representative traces of a leak-subtracted and filtered PIC measured in voltage clamp before (black) and after (red) application of the HCN channel blocker ZD7288 (10 uM; n = 4). ZD7288 did not change the amplitude (K) of the PIC but hyperpolarized the PIC onset voltage (L). Data are presented as individual data points and were analyzed using a paired t-test. P values are reported when significant differences were detected. Hedge’s g values indicated small (0.2-0.49), medium (0.5-0.79) and large (>0.8) effect sizes.

Article Snippet: HCN Channels that underlie the H-current were blocked with ZD7288 (10 uM; Tocris).

Techniques: Membrane, Produced